The far-red fluorescent reactive dye has an excitation maximum of ~633 nm making it ideal for use with the red or HeNe laser with an emission of ~655 nm. Prepare cells for flow cytometric staining using sodium azide-free buffers. You may also like… Yeast Vitality Staining Kit A fluorogenic assay that allows you to determine whether your yeast cells are metabolically active. Quick order. Live/Dead® Fixable Dead Cell Stain Kits For Research Use Only. 4. The LIVE/DEAD™ Fixable Blue Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation. Prepare cells for flow cytometry staining using sodium azide-free buffers. Store at -20°C. In cases where cell fixation is required, we now introduce fixable Zombie Aqua. Yes, Zombie can be used with Annexin V to discriminate live, apoptotic, and dead cells. Fixable Viability Stain 780 Product Information Material Number: 565388 Size: 200 µg Description BD Horizon™ Fixable Viability Stain 780 (FVS780) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. Perfetto SP, Chattopadhyay PK, Lamoreaux L, et al. Procedure. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. Figure 1. Fixable Viability Stain 440UV 200ug × ... We recommend titrating the dye and using the lowest possible concentration that provides adequate resolution of live and dead populations for the cell type of interest to reduce spillover. They irreversibly conjugate to primary amine-containing proteins. Cell Explorer™ Fixable Dead Cell Staining Kit are a set of tools for labeling dead cells for fluorescence microscopic and flow cytometric investigations of cellular functions. Live-or-Dye™ Fixable Viability Stains are cell membrane impermeant amine-reactive dyes. Procedure. Cells double positive for both Zombie and Annexin V are dead, while Zombie-dim/Annexin V-positive cells are apoptotic. DNA-Binding Dyes . The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. Contains 2 vials of LIVE/DEAD® fixable dead cell stain and 500 µL DMSO. Learn more about our tools for live/dead cell discrimination here. DNA-binding dyes, for example 7-AAD and propidium iodide (PI), fluoresce upon intercalation of DNA in dead or dying cells. 10 mL Bovine Calf Serum. Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. 1. Fix - stain with my 1ry Ab - washing step - 2ry Ab - washing step. Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions. Fixable Viability Stain 510 100ug × ... Perfetto SP, Chattopadhyay PK, Lamoreaux L, et al. Figure 1: Schematic representation of live-dead staining with DNA-binding or amine dyes. The assay is based on the reaction of a fluorescent reactive dye with cellular amines. 2. Fixable Viability Stain 780 200ug ... we recommend titrating FVS780 and using the lowest possible dye concentration that provides adequate resolution of live and dead cell populations of interest. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. Live cells will be Zombie-low and Annexin V-negative. Fixable Viability Stain 575V 200ug ... We recommend titrating the dye and using the lowest possible concentration that provides adequate resolution of live and dead populations for the cell type of interest to reduce spillover. Optimal brightness The LIVE/DEAD™ Fixable Red stain was selected based on its fluorescent properties to provide a bright signal when excited with a yellow, green, or blue 488 nm laser. Live/Dead Fixable Stains are cell membrane impermeable amine-reactive dyes. I'm using a LIVE/DEAD™ Fixable Stain Kit from ThermoFisher. 2. The LIVE/DEAD™ Fixable Violet Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation. This dye reacts with and covalently binds to cell-surface and intracellular amines. 1. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. J Immunol Methods. Learn more >> AAT Bioquest. Fixable Viability Stain 575V labeling of cells. In the Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit, Thiazole Orange gives nuclear-concentrated staining in all cells (green), while Live-or-Dye™ 568/583 specifically stains dead cells red (yellow in the merge). Optimal brightness The LIVE/DEAD™ Fixable Blue Stain was selected based on its fluorescent properties to provide a bright signal when excited with a UV laser. Not for use in diagnostic procedures. Cells double positive for both Zombie and Annexin V are dead, while Zombie-dim/Annexin V-positive cells are apoptotic. This dye reacts with and covalently binds to cell-surface and intracellular amines. Since these dyes are unable to pass intact plasma membranes, living cells exclude them … 2 mL 0.4 M EDTA, pH 8. Excitation/Emission: 750⁄775: Form: Solid: Label or Dye: LIVE/DEAD® FixableNear-IR Dead Cell Stain: Product Line: LIVE/DEAD® Solubility: DMSO (Dimethylsulfoxide) Yes, Zombie can be used with Annexin V to discriminate live, apoptotic, and dead cells. These assays are based on the reaction of a fluorescent reactive dye with cellular amines. Live cells will be Zombie-low and Annexin V-negative. Fixable Viability Stain 780 labeling of cells. Cells double positive for both Zombie and Annexin V are dead, while Zombie-dim/Annexin V-positive cells are apoptotic. Adding this fixable Live/Dead dye --> to be able to know the dead cells later. The LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. Amine-reactive dyes for dead cell discrimination in fixed samples. LIVE/DEAD® Fixable Dead Cell Stain Kits Introduction Invitrogen’s LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. Prepare cells for flow cytometry staining using sodium azide-free buffers. Compare LIVE/DEAD Fixable Red Dead Cell Stain Kit, for 488 nm excitation L34971 from Thermo Fisher Scientific on Biocompare.com Live cells will be Zombie-low and Annexin V-negative. Yes, Zombie can be used with Annexin V to discriminate live, apoptotic, and dead cells. This dye reacts with and covale ntly binds to cell-surface and intracellular amines. Contact us. 3. The LIVE/DEAD™ Fixable Far-Red Stain was selected based on its fluorescent properties to provide a bright signal when excited with a red laser. 2. View reference. This kit is used with a red laser flow cytometer. BD Horizon™ Fixable Viability Stain 510 (FVS510) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. S. cerevisiae stained with the Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit: Thiazole Orange to stain all cells (green) and Live-or-Dye™ 568/583 to stain dead cells (red, appears yellow in overlay). Yes, Zombie can be used with Annexin V to discriminate live, apoptotic, and dead cells. These assays are based on the reaction of a fluorescent reactive dye with cellular amines. Do I need a washing step here?! The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. BioLegend provides DNA dyes, Propidium Iodide and 7- AAD, that enter and stain dead cells, but are impermeable to live cells for rapid, cost- effective analysis of unfixed cells. The Zombie live/dead fixable dyes are amine-reactive fluorescent dyes that are impermeant to live cells but permeant to cells with compromised membranes. LIVE/DEAD® Fixable Dead Cell Stain Kits Introduction The LIVE/DEAD ® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. Fixable Viability Stain 440UV labeling of cells. LIVE/DEAD™ Fixable Dead Cell Stain Kits Introduction The LIVE/DEAD ™ Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. Procedure. S. cerevisiae stained with the Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit: Thiazole Orange to stain all cells (green) and Live-or-Dye™ 568/583 to stain dead cells (red, appears yellow in overlay). These assays are based on the reaction of a fluorescent reactive dye with cellular amines. The LIVE/DEAD™ Fixable Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation. Live cells will be Zombie-low and Annexin V-negative. These assays are based on the reaction of a fluorescent reactive dye with cellular amines. The LIVE/DEAD™ Fixable Aqua Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation. Order info. Our ViaQuant™ Blue Fixable Dead Cell Stain Kit is designed for discrimination between live and dead cells during flow cytometry or microscopy. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. 2006; 313(1–2):199-208. 1. The LIVE/DEAD Fixable Dead Cell Stain Kit can be used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. My single-cell preparation is suspended in a solution made up of: 500 mL 1x PBS. As cells die, their plasma membranes become permeable, and DNA is exposed. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Cells double positive for both Zombie and Annexin V are dead, while Zombie-dim/Annexin V-positive cells are apoptotic. Fixable Viability Stain 700 Product Information Material Number: 564997 Size: 100 µg Description BD Horizon™ Fixable Viability Stain 700 (FVS700) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. 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